The Darkside Chronicles is an on-rails shooting game. The game's plot revolves around the personal stories and tragedies in the series, with its main focus on retelling the events of Resident Evil 2 and Resident Evil: Code: Veronica.

A new chapter called Operation Javier fleshes out the plot further and explains the motives of Resident Evil 4 villain Jack Krauser. It takes place in 2002 and is set in the waterside village of Mixcoatl, located in the South American Amparo. It tells the story of Leon S. Kennedy teaming up with Jack Krauser to investigate the connections of crime lord Javier Hidalgo to a former Umbrella researcher.