Best Hits! is one of the many titles in Konami's long-running rhythm game franchise. Notes scroll toward the bottom of the screen along nine tracks, and the player must tap the correct buttons in time with the music to play along. Play well, and the "groove meter" increases, but play poorly and it will drop. If the groove meter is high enough at the end of the song, the player can move on to the next song.

This version includes more than 50 fan-favorite tracks from the first six entries in the series, including several tracks that were cut from the console release of pop'n music 6.