The events of this game take place one year after Professor Layton and Pandora's Box.

Professor Layton and Luke, receive a letter asking for their help sent by... Luke, 10 years later. The professor - believing in a joke by his young assistant - will not attach importance to it but leaves to investigate a week later after the disappearance of Bill Hawks, the Prime Minister of London, in the explosion of a hypothetical machine. go back in time. With the letter telling him to go to a clock shop, Layton and Luke will discover a clock that will take them 10 years into the future. Find an adult Luke but also Flora, Inspector Chelmey and Don Paolo in 165 new riddles and puzzles where Hershel Layton's opponent will be Hershel Layton himself, his future and then his past.