Jack Nicklaus Golf brings Accolade's golf series to the Game Boy with a selection of courses from the computer versions and most features and options also present.

Four courses are available: Jack's Greatest, Castle Pines and Desert Mountain as previously seen in Greatest 18 Holes and Muirfield Village from Unlimited. Gameplay modes are skins (for two to four players) and stroke play (for one to four players). Players can choose their golfer's gender and select from beginner and expert skill levels and different tee modes (professional, men's and women's). Besides playing the complete course, there are also practice options for a single hole, the green, and a driving range.

Playing a hole begins with a top-down overview of the hole, followed by the standard view from behind the golfer. Strokes are done with the standard three-click method: start, power, and accuracy. During play, it is possible to pull up the hole overview, look at the score card and statistics and change the weather conditions. The game also offers replays and mulligans.