As the sequel to 1998's MLB 99, MLB 2000 features an expanded Spring Training mode, new 3D player models with authentic faces and over 175 actual batting stances. Also featured are updated stadiums to reflect details such as the moving fountains at Kauffman Stadium and the outfield monuments at Yankee Stadium, and a new color-commentator in ESPN's Dave Campbell. Returning to the game are the Season (with a 1999 schedule), All-Star Game, Playoffs, Home Run Derby, Exhibition and Spring Training modes of play.

Vin Scully resumes his role as the featured play-by-play announcer and the game includes both Major League Baseball and MLBPA licenses for real teams and players (with ratings based on the 1998 season). Up to 24 athletes can be created and stored to a memory card with each player receiving 236 points to distribute among seven categories: running, arm, fielding, reaction, clutch, power and batting average. Now if you decide to take part in the Spring Training mode, your created player will have only seven points initially available. Your goal in this mode is to reach fifty points in order to earn a spot on the 25-man Major League roster.

Once in the big leagues, your player will have to keep performing at a Major League level or he'll be sent back down to the minors. Yet if your player manages to earn 100 points, he'll be penciled in the starting lineup of an All-Star team! MLB 2000 also offers you the chance to manage games (the computer carries out the action), realign teams in each division, sign free agents, call up farm system prospects, trade players, enter a 25-round draft against the computer and create user records.

Options include three levels of difficulty, wind, injuries, adjustable innings, five batting views, three fielding views, day or night games, errors, alternate uniforms and using either a cursor or timing system for hitting. One to six blocks of memory card data is needed to save games.